the antibiotic concentration

Introduction
When would we want to determine the antibiotic concentration ie quantify the level of antibiotic present?
Clinical
Food eg milk
Pharmaceutical products (potency and stability)
What samples would you test?
How does this relate to treatment of patients with antibiotics?
Materials and Methods
Brief outline of the procedure
identify the organism, medium and antibiotic
0 NOT reproduce exactly what is in the laboratory manual
Write in past tense

Plot log 10 antibiotic concentration against the zone diameter in mm.
Draw line of best fit
For the concentrations used the standard curve should be a straight line.
Read the concentrations (log10 ) of the unknown antibiotic solution from the graph
Convert the log value to whole number
(Note: value of diluted solution should be approximately ‘/2the unknown X.)
Discussion
Accuracy of your results
If you did not achieve the
correct result discussthe reasons for the error
Any problems you encountered.
Sources of variation/errors in the method
preparation of dilutions and factors that may affect the zone diameter

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