HPLC

Course Title: Biomedical Science
Unit Title: Analytical Techniques in Biomedical Science

1Houre writ up exam to answer this Question (use essay format)
QUESTON- Describe the principle of chromatographic separation with special reference to high performance liquid chromatography (HPLC). Your answer should include a description of the component and their function in a typical HPLC system?
-Definition
*Chromatography
*HPLC
– Diagram…..Components
*Mobil-stationary …..Phases
*Affinity
*Elution volume
*Retention time
*Principle of CHR
* Resolution
*Normal or reverse…..Phases (polar –nonpolar)
*Detector
*Injection sample (How many types)
-How can measure attention time?
-Culprit the calm internal STD (standard)
-Why we need to measure the standard?
– Application of (HPLC)
-How to calculate the result?

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