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OYE-mutant, Clone #566 and Ax2 wild-type D. discoideum exhibit different developmental stages in starving conditions
Follow these Directions for the rough draft thesis:
1. Introduction: Begin looking at the literature related to these genes. Write two paragraphs
about these genes. Based on the literature, write a hypothesis for each gene for each
experiment/assay. Introduce your reader to the three major ways we are characterizing the
mutants Development, Phagocytosis, Microscopy with Actin, DAPI stains.
note* i will upload a file with the background information for the model Dictystelium
Discoideum, which is the the model organism i use in the experiments, so add this
information in this section. This section should cite at least four primary sources from
the scientific literature.
2. Methods :Write two good paragraphs on each mutant. Cite the literature papers from
dictybase.org in the methods section. note* i will upload a methods file with the 3
assays/experiments I used. I will upload the literature paper from dictybase.org
3. Results:For the results make connections with the (development data, Phagocytosis data,
Fluorescence microscopy with actin/DAPI stains data) by writing two sentence statement of
results for each assay/experiment create figures and legends (use arrows on the images) For
each gene make a list of ;The figures that you want to present(my writer can pick for me)there
should be one for each major assay/experiment. note* i will upload all images so you can
create figures and legends. Compare and contrast each image.
4. Discussion: For discussion Write a two sentence statement of your conclusions for each
gene for each assay/experiment. Write a statement of how the conclusions matched your
original hypothesis.
Explain how molecular biology looks forward to work in the future with this whole experiment
process.
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